Kit for detecting goldfish haematopoietic necrosis virus and application thereof

检测金鱼造血器官坏死病病毒的试剂盒及其应用

Abstract

本发明提供了用于检测金鱼造血器官坏死病病毒试剂盒。本发明首先提供了用于检测金鱼造血器官坏死病病毒的实时荧光定量PCR引物和探针,其序列分别如SEQ?ID?NO2,SEQ?ID?NO3和SEQID?NO4所示。本发明还提供了使用上述引物和探针进行荧光定量PCR检测金鱼造血器官坏死病病毒的方法。本发明的检测方法及其检测试剂盒具有检测准确,灵敏度高、特异性强,简便快速的优点,具有良好的临床标本检测能力。
The invention provides a kit for detecting goldfish haematopoietic necrosis virus. The invention provides a real-time fluorescence quantitative PCR (Polymerase Chain Reaction) primer and a probe for detecting goldfish haematopoietic necrosis virus at first, wherein the sequences are respectively represented by SEQ ID NO2, SEQ ID NO3 and SEQ ID NO4. The invention further provides a method for detecting goldfish haematopoietic necrosis virus by using the primer and the probe through fluorescence quantitative PCR. The detection method and the detection kit disclosed by the invention have the advantages of being accurate to detect, high in sensitivity and specificity, simple, convenient and rapid, and have good clinical sample detection capability.

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