The invention relates to a toxicity attenuation agent. The toxicity attenuation agent comprises, according to proportional contents, one sheet of coptis chinensis, one ginkgo nut, 6 g of moxa, 8 g of honeysuckle, eight sheets of longjing tea, 6 g of folium artemisiae argyi or moxa, 6 g of loofah sponge and one piece of liquorice. A preparation method of the toxicity attenuation agent includes the preparation processes that all ingredients are mixed in sequence according to proportional contents; then the mixture is placed into two layers of organic cotton cloth without dying; the organic cotton cloth with the mixture is placed into wooden ware containing 6 kg or 8 kg of bactericide-free water for soaking, a medicine capsule sinks into water, the object is fished out after thirty minutes, four times of clockwise rotation, four times of anticlockwise rotation and six times of clockwise rotation are sequentially carried out with the hand in a solution, a medicine is relatively and evenly distributed in the water, and then the solution is injected into related packaging materials. The toxicity attenuation agent has the beneficial effects that compared with real ingredients, the toxicity attenuation agent is a non-genetically modified organic product and a non-radioactivity breeding product, person cultivation can be effectively carried out, and a good role is played in toxicity attenuation. Toxicity attenuation is achieved, and harmful bacteria such as germs and viruses are relatively converted into beneficial bacteria.