Method for extracting, separating and detecting active ingredients in safflower carthamus medicinal material

一种红花药材中活性成分的提取、分离及其检测方法

Abstract

本发明涉及一种红花药材中活性成分的提取、分离及其检测方法,所述的方法包括:⑴提取过程:取红花药材,用乙醇提取,醇提液进行浓缩干燥,加入酸水水解,水解液回收溶剂并浓缩,再用乙酸乙酯萃取,萃取液浓缩、干燥成浸膏,留用;⑵分离、纯化过程:取步骤⑴中留用的浸膏,用硅胶柱分离,再用葡聚糖凝胶LH-20柱纯化,即得。本发明所述的提取、分离和检测方法具有简单可行、科学等特点。
The invention relates to a method for extracting, separating and detecting active ingredients in a safflower carthamus medicinal material. The method comprises the following steps: (1) extracting, namely taking the safflower carthamus medicinal material, extracting by using ethanol, concentrating and drying the alcohol extract, adding acid water for hydrolysis; recovering a solvent from the hydrolysate and concentrating; extracting by using ethyl acetate; and concentrating and drying the extracting solution into extract for later use; and (2) separating and purifying, namely taking the extract prepared in the step (1), separating by using a silica gel column, purifying by using a dextrangel LH-20 column, thus obtaining the active ingredients in the safflower carthamus medicinal material. The extraction, separation and detection method provided by the invention has the characteristics of simplicity, feasibility, scientificity and the like.

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