Method for extracting, separating and detecting active ingredients in safflower carthamus medicinal material



The invention relates to a method for extracting, separating and detecting active ingredients in a safflower carthamus medicinal material. The method comprises the following steps: (1) extracting, namely taking the safflower carthamus medicinal material, extracting by using ethanol, concentrating and drying the alcohol extract, adding acid water for hydrolysis; recovering a solvent from the hydrolysate and concentrating; extracting by using ethyl acetate; and concentrating and drying the extracting solution into extract for later use; and (2) separating and purifying, namely taking the extract prepared in the step (1), separating by using a silica gel column, purifying by using a dextrangel LH-20 column, thus obtaining the active ingredients in the safflower carthamus medicinal material. The extraction, separation and detection method provided by the invention has the characteristics of simplicity, feasibility, scientificity and the like.




Download Full PDF Version (Non-Commercial Use)

Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
    CN-1403080-AMarch 19, 2003中国人民解放军第二军医大学Application of kaempferol and its derivative in preparing medicine for cardiac and cerebral vascular diseases

NO-Patent Citations (2)

    BAUTISTA, ELIHU ETC.: "Antiprotozoal activity of flavonoids isolated from Mimosa tenuiflora (Fabaceae-Mimosoideae)", 《JOURNAL OF THE MEXICAN CHEMICAL SOCIETY》, vol. 55, no. 4, 18 November 2011 (2011-11-18), pages 251 - 253
    E.N.NOVRUZOV ETC.: "anthocyans of carthamus species", 《CHEMISTRY OF NATURAL COMPOUNDS》, vol. 34, no. 4, 30 July 1998 (1998-07-30), pages 557 - 558

Cited By (0)

    Publication numberPublication dateAssigneeTitle