Production of acid soluble soy protein isolates ("s700")

酸溶性大豆蛋白分离物(“s700”)的制备

Abstract

A soy protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably an isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is formed by extracting a soy protein source with a salt solution, preferably aqueous sodium chloride solution, to form an aqueous protein solution having a pH of about 1.5 to 11, preferably about 5 to about 7 and separating the resulting aqueous protein solution from residual soy protein source. The protein concentration of the aqueous protein solution is increased to about 50 to about 400 g/L while the ionic strength is maintained substantially constant by using a selective membrane technique. The resulting concentrated protein solution is optionally diafiltered and a calcium salt, preferably calcium chloride, is added to the concentrated and optionally diafiltered protein solution to a conductivity of 15 to about 85 mS. Precipitate formed as a result of the calcium salt addition is removed and the resulting clarified retentate is diluted into about 2 to about 20 volumes of water prior to acidification to a pH of about 1.5 to about 4.4 to produce an acidified clear protein solution. The acidified clear protein solution is then concentrated and optionally diafiltered and optionally dried. Variations of this procedure can be used to produce a soy protein product which is soluble, transparent and heat stable in acidic aqueous environments.
一种蛋白含量为至少约60重量%(N×6.25)d.b.的大豆蛋白产品,优选蛋白含量为至少约90重量%(N×6.25)d.b.的分离物,其通过以下形成:将大豆蛋白源用盐溶液,优选氯化钠水溶液提取,以形成具有约1.5-11,优选约5-约7的pH的蛋白水溶液,并将所得蛋白水溶液与残留大豆蛋白源分离。将蛋白水溶液的蛋白浓度增加至约50-约400g/L,同时用选择性膜技术维持离子强度基本不变。将所得浓缩蛋白溶液任选渗滤,将钙盐,优选氯化钙加入浓缩和任选渗滤的蛋白溶液至15-约85mS的电导率。除去由于加入钙盐而形成的沉淀物,将所得澄清保留物稀释在约2-约20体积水中,然后酸化至约1.5-约4.4的pH,以制备酸化澄清的蛋白溶液。然后将酸化澄清的蛋白溶液浓缩和任选渗滤和任选干燥。该程序的变化可用于制备在酸性含水环境中可溶解,透明和热稳定的大豆蛋白产品。

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NO-Patent Citations (1)

    Title
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